Barnes Maze


The Barnes Maze is a rodent apparatus for memory and spatial learning in mice and rats. The test provides information about hippocampus function.

The maze consists of an elevated platform with 40 equally spaced holes around the perimeter. Visual cues are placed around the platform along with randomly placed escape tunnels.

To learn more about the maze, hippocampus, or learning and memory–use the references listed below!


  1. Pitts M. W. (2018). Barnes Maze Procedure for Spatial Learning and Memory in MiceBio-protocol8(5), e2744. doi:10.21769/bioprotoc.2744.
  2. Van Den Herrewegen, Y., Denewet, L., Buckinx, A. et al. The Barnes Maze Task Reveals Specific Impairment of Spatial Learning Strategy in the Intrahippocampal Kainic Acid Model for Temporal Lobe Epilepsy. Neurochem Res (2019) 44: 600.
  3. Gawel, K., Gibula, E., Marszalek-Grabska, M., Filarowska, J., & Kotlinska, J. H. (2019). Assessment of spatial learning and memory in the Barnes maze task in rodents-methodological considerationNaunyn-Schmiedeberg’s archives of pharmacology392(1), 1–18. doi:10.1007/s00210-018-1589-y.

Additional information


Mouse, Rat


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