Square chambers are used for locomotion tests, gait analysis, and novel object recognition tests as an open field where novel objects are strategically placed in opposite corners of one another. Square chambers are typically used for NORT, alternatively to circular/round chambers.

In humans, recognition memory is tested with a visual comparison where an object and information may be mismatched in order to test proficiency at detecting the errors.

In rodents, recognition memory is assessed using novel object recognition tests and delayed matching/nonmatching to sample tests (DMST OR DNMST). These tests originated in the 1970s and have progressed to the novel behavioral tests that exist today.