Flow Cytometry CRO

Flow Cytometry CRO

Embark on a transformative journey in immunology research and drug development with Anilocus’s advanced Flow Cytometry Services. Flow cytometry, a powerful and versatile analytical technique, lies at the heart of our commitment to precision and innovation.

Let’s explore the process of flow cytometry and the features that make it an indispensable tool.

The Flow Cytometry Process

Flow cytometry is a technique that allows the simultaneous analysis of multiple physical and chemical characteristics of single cells as they flow in a fluid stream through a laser beam. Here’s a glimpse into the process:

  1. Cell Suspension: Cells are carefully prepared into a single-cell suspension, ensuring a homogeneous sample for analysis.
  2. Fluorescent Labeling: Cells are tagged with fluorescent labels, typically antibodies, specific to cell surface markers or intracellular components of interest.
  3. Laser Excitation: The cell suspension passes through a flow cell where it intersects with laser beams, causing the fluorescent labels to emit light.
  4. Scattering and Emission: The emitted light, both forward and side scatter, and fluorescent emissions are detected by photomultiplier tubes, generating signals for each cell.
  5. Data Acquisition and Analysis: Sophisticated software processes the signals, allowing the generation of multidimensional data for each cell, including size, granularity, and fluorescence intensity.
  6. Cell Sorting (Optional): In flow cytometry, particularly with Fluorescence-Activated Cell Sorting (FACS), cells of interest can be physically separated based on their fluorescence characteristics.

Useful Features of Flow Cytometry

  • Multiparametric Analysis: Simultaneous measurement of multiple parameters provides a comprehensive view of cellular characteristics in a single experiment.
  • Cell Subset Identification: Precise identification and characterization of specific cell populations based on surface markers or intracellular components.
  • Quantitative Analysis: Accurate quantification of biomarkers, allowing for the assessment of protein expression levels or cell proliferation rates.
  • Functional Insights: Intracellular staining enables the assessment of functional activities, such as cytokine production, providing insights into cellular responses.
  • Cell Sorting Precision: Fluorescence-Activated Cell Sorting allows for the isolation of specific cell populations with high purity, facilitating downstream applications.
  • High-Throughput Capability: Rapid analysis of thousands of cells per second accelerates data acquisition and enhances the efficiency of experiments.
  • Biomarker Profiling: Customized panels enable the profiling of disease-specific biomarkers, offering valuable insights for diagnostic and therapeutic strategies.

Flow cytometry at Anilocus goes beyond mere analysis; it’s a dynamic exploration of cellular profiles, providing a deeper understanding of immune responses and paving the way for innovative drug development.

Contact Us!

Contact us at Anilocus, where the pursuit of scientific excellence converges with a steadfast commitment to supporting the biopharmaceutical industry:

Sony LE-SA3800EA Spectral Cell Analyzer on display.

    Significance of Flow Cytometry in Drug Development


    Safety Assessment

    Early detection limits risks, ensuring patient safety in clinical trials.


    Efficacy Enhancement

    In-depth studies at Anilocus refine drug candidates, enhancing effectiveness.


    Precision Medicine

    Biomarkers enable tailored strategies and interventions with patient profiles.


    Regulatory Compliance

    Comprehensive data aids submissions, expediting approvals through regulatory compliance.

    Our Case Studies