Dental and Orofacial Disorders

Dental and Orofacial Disorders

In Vivo Preclinical CRO Services for Oral & Dental Medicine

In the industry of oral and dental medicine, preclinical research is an essential step towards developing new treatments and therapies. However, conducting this research can be a daunting and time-consuming task. That’s where we come in. Anilocus provides dental medicine CRO services for in vivo safety and efficacy assessments.

We offer a wide range of solutions to help streamline the preclinical research process, from study design to data analysis.

With our expertise in pharmacology and toxicology, our preclinical solutions can provide valuable insights into your novel treatment.

At AniLocus, we offer in vivo contract research services in multiple animal models for safety and efficacy assessments.

Contact Us! Learn more about our preclinical CRO services:

Accelerate Dental Drug Development with Our Cutting-Edge Research Services

Take a closer look at our preclinical CRO services for oral and dental medicine listed below and explore how we can help accelerate the drug development process while ensuring the highest standards of quality and safety:

  • Bone Regeneration Therapeutics
  • Cell-based therapies (MSCs)
  • Dental Biomaterials
  • Dental Combination Drug Products
  • Dental Device Testing
  • Dental Drug Formulation & Drug Delivery
  • Dental Extraction

Frequently Asked Questions about in vivo studies for dental medicine:

When selecting an animal model for a dental disease study, it is important to consider the characteristics that best imitate the disease in humans.

For example, if the study involves periodontal disease, an animal model species with similar anatomy, physiology, and metabolic characteristics as humans would be ideal. It is also important to consider the cost of the animal species, the availability of tissue samples, post-processing of tissue samples, and the ease of handling and manipulating the animal for experiments.

The exact timeline for in vivo studies of dental disease in small laboratory animals depends on the research goals and the specific type of dental disease being studied. Generally speaking, the timeline may include the following stages:

  • Initial Cohort Screening
  • Pre-experimental Procedures; acclimation to the laboratory environment
  • Dental Imaging
  • Experimental Treatments
  • Clinical Scoring
  • Post-Experimental Assessments; Plaque Index and Histology, etc.

The timeline for each stage can vary significantly depending on the research objectives and the type of dental disease being studied. Ultimately, the study timeline will be designed to maximize experimental outcomes.

The estimated cost to run in vivo animal studies for dental diseases can vary significantly depending on the type of study, the animal species used, the number of animals, and other factors.

Generally, costs for such studies can range from tens of thousands to hundreds of thousands of dollars.

Biomaterials used in dental medicine for in vivo animal studies at Anilocus can vary widely and depend on the type of study being conducted and the species selected. For most translational studies, common biomaterials used in these types of studies include biocompatible polymers, ceramics, metals, and composites.

In addition, natural materials like nanocellulose, collagen, and chitosan have been used to create scaffolds for human tissue engineering. Furthermore, bioinks/3D derived from alginate (printable alginate), hyaluronic acid, and fibrin have been used for 3D printing of biomaterials for dental applications.

In vivo testing for dental disease can involve various drug modalities, such as antibiotics, anti-inflammatory medications, and dental anesthetics and analgesics. Depending on the type of dental disease being tested for, other modalities may be used to help gain a better understanding of the disease and its response to these medications. For example, a combination of antibiotics and antifungals may be employed to treat a periodontal infection.

Additionally, certain forms of topical or systemic treatments may also be tested, such as fluoride or chlorhexidine gels. It is important to note that the specific types of drug modalities used will depend on the severity of the dental disease being treated and the necessary endpoints for analysis.

Live imaging techniques are valuable for conducting in vivo dental disease studies in mice, as they enable researchers to investigate biological processes in real time.

For example, assessing periodontitis and dental caries. Fluorescence imaging allows researchers to image autofluorescent molecules that are present in dental tissues, while bioluminescence imaging enables the detection of light emitted by cells expressing a bioluminescent reporter gene. Magnetic resonance imaging can provide detailed images of the structure and composition of dental tissues, such as mineralized structures and soft tissues.

Using animal and human cells and tissue we can perform multiple interrogative assays depending on the therapeutic area, medical device, and/or sample type and necessary endpoints:

  • DNA: Extraction, PCR, ddPCR, qPCR, sequencing, genotyping
  • RNA: Gene expression profiling, RT-PCR, miRNA, Next-generation sequencing (Nanostring, Illumina), spatial transcriptomics, In situ hybridization (ISH), Multiplex ISH
  • Protein: Immunoassays (ELISA, EIA, Luminex), proteomics, ligand binding assays, protein expression, immunohistochemistry (IHC), immunocytochemistry (ICC), and  immunofluorescence (IF)